Archives
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HyperScript RT SuperMix for qPCR in RNA Analysis
2026-08-24
HyperScript RT SuperMix for qPCR is a 5X premixed reverse transcription solution for two-step qRT-PCR. Its thermally stable HyperScript Reverse Transcriptase and mixed-primer design address structurally complex RNA and low-input workflows without implying that reverse transcription alone validates a biological mechanism.
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Methyl-β-cyclodextrin: Practical Lab Guide
2026-08-24
Methyl-β-cyclodextrin (SKU C6939) provides a controllable research reagent for membrane cholesterol extraction and related studies of membrane organization. It is intended for laboratory workflows only, not diagnostic, therapeutic, or medical use, and solutions should be prepared for prompt use rather than long-term storage.
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Doxorubicin Workflows for Cancer Research
2026-08-23
Doxorubicin, also called Adriamycin, provides a versatile reference system for DNA damage, apoptosis, chromatin remodeling, and combination-response studies. This practical guide connects a controlled cell-culture workflow with clinically informed assay design, while emphasizing exposure control, orthogonal validation, and troubleshooting.
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MRT68921: A Practical ULK1 Kinase Inhibitor Guide
2026-08-22
MRT68921 enables controlled, proximal blockade of autophagy initiation rather than the pathway activation produced by rapamycin. This guide translates its ULK1/2 activity into practical workflows for ATG13 phosphorylation, LC3 flux, and lipid-autophagy studies, with controls that improve mechanistic confidence.
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PDHA1 Succinylation, α-KGA, and Cholangiocarcinoma
2026-08-22
A 2025 Nature Communications study identifies PDHA1 lysine 83 succinylation as a metabolic switch that increases α-ketoglutaric acid in the cholangiocarcinoma microenvironment. The resulting OXGR1–MAPK signal suppresses macrophage MHC-II antigen presentation, while inhibiting succinylation with CPI-613 may improve gemcitabine–cisplatin sensitivity.
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Sanggenol L Drives Ferroptosis in NSCLC via miRNA–p53
2026-08-21
The reference study identifies sanggenol L as an inducer of ferroptosis in non-small cell lung cancer (NSCLC) and connects this effect to the miR-26a-1-3p/MDM2/p53/SLC7A11 regulatory axis. Its combination of molecular perturbation, ferroptosis-associated readouts, xenograft studies, and patient-derived tumor xenografts provides a mechanistic framework for evaluating natural-product strategies against NSCLC.
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From PS Exposure to Translational Apoptosis Insight
2026-08-20
A mechanistic and strategic guide to using the Annexin V-Cy5/DAPI Apoptosis Kit to connect phosphatidylserine exposure with P2RX1-driven mitochondrial apoptosis, tyrosine kinase inhibitor response, and more rigorous translational decision-making in Ph+ ALL research.
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5-Azacytidine Workflow for Cancer Epigenetics
2026-08-20
Build reproducible 5-Azacytidine assays that connect DNMT inhibition with gene reactivation, cytotoxicity, and cancer-cell dormancy. This workflow translates the AZA–retinoic acid findings from a metastasis study into practical dose, timing, control, and troubleshooting decisions.
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Cefepime (BMY-28142) in Resistance Models
2026-08-19
Cefepime (BMY-28142) supports a practical bridge between β-lactam susceptibility testing, plasmid-mediated resistance analysis, and central nervous system infection research. This guide shows how to build reproducible assays while treating resistance, blood-brain barrier exposure, and neurotoxicity as separate experimental questions.
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5-Azacytidine: DNMT Inhibitor for Cancer Research
2026-08-19
5-Azacytidine, also called 5-AzaC, is a cytosine analogue DNA methyltransferase inhibitor used to study DNA demethylation, gene reactivation, and cancer-cell responses. Its mechanism depends on nucleic-acid incorporation and DNMT trapping, while recent work shows that combination treatment with retinoic-acid agonists can reprogram disseminated cancer cells into dormancy.
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CFDA SE Cell Tracer Kit: Practical Workflow
2026-08-18
The CFDA SE Cell Tracer Kit provides stable fluorescent labeling for cell lineage tracing, cell proliferation studies, and related flow cytometry or microscopy workflows. It is intended for persistent covalent tracking over several days, not for short-term reversible labeling or rapid real-time dye-clearance studies.
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Biotin-tyramide (A8011): Practical TSA Guide
2026-08-18
Biotin-tyramide (A8011) supports HRP-driven biotin deposition when conventional immunohistochemistry or in situ hybridization signals are too weak for reliable imaging. It is intended for fixed cells and tissue sections in research workflows, not as a general water-soluble labeling reagent or for diagnostic and medical use.
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Cy3 Goat Anti-Rabbit IgG: From Biomarker to Image
2026-08-17
Discover how the Cy3 Goat Anti-Rabbit IgG (H+L) Antibody can connect quantitative HMGB1 biomarker discovery with spatial validation in immunofluorescence, IHC, and ICC. This evidence-driven guide focuses on assay architecture, controls, signal interpretation, and practical limitations rather than routine product promotion.
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HDAC Inhibition Reverses EBV-Driven NPC Dedifferentiation
2026-08-17
The reference study identifies an LMP1–STAT5A–HDAC1/2–CEBPA pathway through which Epstein–Barr virus promotes dedifferentiation and plasticity in nasopharyngeal carcinoma. Its experiments support HDAC inhibition as a differentiation-oriented strategy for restoring CEBPA activity and reducing stem-like tumor-cell states in xenograft models.
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CHIR-99021 for Mesodermal Organoid Signaling
2026-08-16
CHIR-99021 (CT99021) gives stem cell and organoid researchers a tunable way to interrogate GSK-3–dependent Wnt signaling, lineage commitment, and tissue organization. This workflow connects a defined small-molecule pulse with the budoid model’s epithelial–mesodermal signaling logic, while providing practical controls for dose, timing, stock handling, and morphological variability.